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Peptide Separations Reversed-Phase Bio Columns (3)
Intact & Subunit Analysis (3)
Charge Variant Analysis (3)
Antibody Titer Determination (2)
Aggregate & Fragment Analysis (2)
Applications and Industries
Pharma & Biopharma Applications (9)
Proteomics (6)
Biopharma & Pharma Applications (3)
Biologics & Biosimilars (3)
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Content Type
LC (11)
GPC (3)
SEC (3)
UHPLC (1)
Bio SEC-3 (2)
Bio Mab NP 5 (2)
Bio-Monolith Protein A (2)
AdvanceBio Peptide Map (2)
ZORBAX 300SB-C3 (1)
IgG1 (6)
mAb (3)
lysozyme (2)
cytochrome c (2)
ribonuclease a (2)
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必威体育登录手机Agilent 1290 Infinity (6)
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Applications
Combining small-scale purification and analysis of monoclonal antibodies on one instrument (PDF)

必威体育登录手机Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.

  • Publication Part Number: 5991-1195EN
  • Created: 01 May 2016
  • 854 KB
  • PDF
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Publication Part Number 5991-1195EN
Analysis Analysed with Part No.
Bio Mab
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
DAD
Sample Chemical Group
monoclonal antibodies
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Analysis of Monoclonal Antibody (mAb) Using Agilent 1290 Infinity LC System Coupled to Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) (PDF)

This work describes a high flow LCMS method to demonstrate the high resolution mass analysis of mAbs with good chromatography and its utility in analysis of Biosimilar mAbs.

  • Publication Part Number: 5991-4266EN
  • Created: 01 July 2014
  • 310 KB
  • PDF
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Publication Part Number 5991-4266EN
Analysis Analysed with Part No.
Poroshell 300SB-C8
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6530
Analytical Technique Technique
LC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures (PDF)

In this application note, pre-packed Bio-monolith Protein A columns, part of the AdvanceBio family, were used to demonstrate the quick capture of monoclonal antibody titer

  • Publication Part Number: 5991-2990EN
  • Created: 08 Jan 2014
  • 441 KB
  • PDF
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Publication Part Number 5991-2990EN
Analysis Analysed with Part No.
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
Sample Matrix
cell culture
Example category image description
Applications
Fast and Efficient Peptide Mapping of a Monoclonal Antibody (mAb): UHPLC Performance with Superficially Porous Particles (PDF)

必威体育登录手机The Agilent AdvanceBio Peptide Mapping column separates mAb tryptic digests at low system pressures, providing UHPLC performance from HPLC operating conditions.

  • Publication Part Number: 5991-3585EN
  • Created: 06 Dec 2013
  • 2 MB
  • PDF
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Publication Part Number 5991-3585EN
Analysis Analysed with Part No.
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6530
Analytical Technique Technique
LC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
peptides
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机High Resolution and Rapid Peptide Mapping of Monoclonal Antibody Using an Agilent 1290 Infinity UHPLC and an Agilent 6550 iFunnel Q-TOF LC/MS System (PDF)

Peptide mapping is the common analytical technique for characterizing biologic therapeutic proteins.

  • Publication Part Number: 5991-3600EN
  • Created: 22 Nov 2013
  • 1 MB
  • PDF
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Publication Part Number 5991-3600EN
Analysis Analysed with Part No.
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6550
Analytical Technique Technique
LC
UHPLC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
peptides
proteins
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Analysis of Intact and C-terminal Digested IgG1 on an Agilent Bio MAb NP5 Column (PDF)

必威体育登录手机Agilent Bio MAb columns, in an Agilent 1260 Infinity Bio-inert Quaternary LC, characterize the charge heterogeneity of IgG1monoclonal antibody...

  • Publication Part Number: 5991-0895EN
  • Created: 01 Aug 2013
  • 240 KB
  • PDF
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Publication Part Number 5991-0895EN
Analysis Analysed with Part No.
Bio Mab NP 5
Instrument
必威体育登录手机Agilent 1100
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
bio-therapeutics
monoclonal antibodies
Sample Matrix
chinese hamster
Example category image description
Applications
pH Gradient Elution for Improved Separation of Monoclonal Antibody Charge Variants (PDF)

Ion exchange is a very useful technique to separate protein mixtures under mild conditions, most commonly by applying a shallow gradient of increasing salt concentration as el

  • Publication Part Number: 5990-9629EN
  • Created: 01 Aug 2013
  • 1 MB
  • PDF
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Publication Part Number 5990-9629EN
Analysis Analysed with Part No.
Bio Mab NP 5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Reversed-Phase Optimization for Ultra Fast Profiling of Intact and Reduced Monoclonal Antibodies using Agilent ZORBAX Rapid Resolution High Definition 300SB-C3 Column (PDF)

必威体育登录手机Rapid reversed-phase separations of intact and reduced monoclonal antibodies (mAbs) were optimized using Agilent ZORBAX RRHD 300SB-C3 columns.

  • Publication Part Number: 5990-9667EN
  • Created: 13 Feb 2012
  • 364 KB
  • PDF
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Publication Part Number 5990-9667EN
Analysis Analysed with Part No.
ZORBAX 300SB-C3
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Ultra High Speed and High Resolution Separations of Reduced and Intact Monoclonal Antibodies with Agilent ZORBAX RRHD Sub-2 μm 300 Diphenyl UHPLC Column (PDF)

JM

  • Publication Part Number: 5990-9668EN
  • Created: 07 Feb 2012
  • 807 KB
  • PDF
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Publication Part Number 5990-9668EN
Analysis Analysed with Part No.
ZORBAX 300-Diphenyl
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
Sample Matrix
chinese hamster ovary
test mixture
Example category image description
Applications
必威体育登录手机Fast Separation of Monoclonal Antibody and Dimer by SEC with Agilent Bio SEC (PDF)

The aggregation of protein therapeutics has become a major concern for the

  • Publication Part Number: 5990-8613EN
  • Created: 02 Aug 2011
  • 602 KB
  • PDF
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Publication Part Number 5990-8613EN
Analysis Analysed with Part No.
Bio SEC-3
Bio SEC-3 300A
Instrument
---
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Characterization of monoclonal antibodies on the Agilent 1260 Infinity Bio-inert Quaternary LC by Size Exclusion Chromatography using the Agilent BioSEC columns (PDF)

必威体育登录手机Application note for the characterization of monoclonal antibodies on the Agilent 1260 Infinity Bio-inert Quaternary LC by SEC using the Agilent BioSEC columns

  • Publication Part Number: 5990-6414EN
  • Created: 01 Feb 2011
  • 996 KB
  • PDF
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Publication Part Number 5990-6414EN
Analysis Analysed with Part No.
Bio SEC-3
Bio SEC-5
Instrument
必威体育登录手机Agilent 1200
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
DAD
Sample Chemical Group
antibodies
monoclonal antibodies
Sample Matrix
test mixture

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