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Peptide Separations Reversed-Phase Bio Columns (4)
Intact & Subunit Analysis (4)
Antibody Titer Determination (2)
Charge Variant Analysis (1)
N-Glycan Sample Preparation & Analysis (1)
Applications and Industries
Pharma & Biopharma Applications (8)
Proteomics (5)
Biologics & Biosimilars (4)
Biopharma & Pharma Applications (3)
Pharmaceutical Small Molecules (1)
Content Type
LC (9)
LC/MS (1)
GPC (1)
SEC (1)
UHPLC (1)
ZORBAX RRHD 300-DP (2)
Bio-Monolith Protein A (2)
AdvanceBio Peptide Map (2)
ZORBAX 300SB-C3 (1)
Poroshell 300SB-C8 (1)
monoclonal antibodies (6)
proteins (4)
peptides (2)
antibodies (2)
glycans (1)
IgG1 (4)
lysozyme (2)
cytochrome c (2)
mAb (2)
ribonuclease a (2)
MS-QTOF (3)
UV (2)
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Example category image description
Applications
Combining small-scale purification and analysis of monoclonal antibodies on one instrument (PDF)

必威体育登录手机Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.

  • Publication Part Number: 5991-1195EN
  • Created: 01 May 2016
  • 854 KB
  • PDF
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Publication Part Number 5991-1195EN
Analysis Analysed with Part No.
Bio Mab
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
DAD
Sample Chemical Group
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
Sensitive and Reproducible Glycan Analysis of Human Immunoglobulin G (PDF)

This Application Note demonstrates the sensitive and reproducible analysis of N-linked glycans of human immunoglobulin G with a novel HILIC column and fluorescence detection.

  • Publication Part Number: 5991-4801EN
  • Created: 01 Nov 2015
  • 840 KB
  • PDF
Quick View
Publication Part Number 5991-4801EN
Analysis Analysed with Part No.
AdvanceBio Glycan Mapping
Instrument
必威体育登录手机Agilent 1260 Infinity
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
FLD
Sample Chemical Group
antibodies
glycans
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Analysis of Monoclonal Antibody (mAb) Using Agilent 1290 Infinity LC System Coupled to Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) (PDF)

This work describes a high flow LCMS method to demonstrate the high resolution mass analysis of mAbs with good chromatography and its utility in analysis of Biosimilar mAbs.

  • Publication Part Number: 5991-4266EN
  • Created: 01 July 2014
  • 310 KB
  • PDF
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Publication Part Number 5991-4266EN
Analysis Analysed with Part No.
Poroshell 300SB-C8
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6530
Analytical Technique Technique
LC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Reducing Cycle Time for Quantification of Human IgG Using the Agilent Bio-Monolith Protein A HPLC Column (PDF)

必威体育登录手机This Application Note describes the quantification of human IgG with the Agilent Bio-Monolith Protein A HPLC Column with alternating column regeneration.

  • Publication Part Number: 5991-4723EN
  • Created: 01 June 2014
  • 836 KB
  • PDF
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Publication Part Number 5991-4723EN
Analysis Analysed with Part No.
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
antibodies
示例矩陣
test mixture
Example category image description
Applications
Fast and Efficient Peptide Mapping of a Monoclonal Antibody (mAb): UHPLC Performance with Superficially Porous Particles (PDF)

必威体育登录手机The Agilent AdvanceBio Peptide Mapping column separates mAb tryptic digests at low system pressures, providing UHPLC performance from HPLC operating conditions.

  • Publication Part Number: 5991-3585EN
  • Created: 06 Dec 2013
  • 2 MB
  • PDF
Quick View 必威体育赛事投注Supporting Products
Publication Part Number 5991-3585EN
Analysis Analysed with Part No.
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6530
Analytical Technique Technique
LC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
peptides
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机High Resolution and Rapid Peptide Mapping of Monoclonal Antibody Using an Agilent 1290 Infinity UHPLC and an Agilent 6550 iFunnel Q-TOF LC/MS System (PDF)

Peptide mapping is the common analytical technique for characterizing biologic therapeutic proteins.

  • Publication Part Number: 5991-3600EN
  • Created: 22 Nov 2013
  • 1 MB
  • PDF
Quick View 必威体育赛事投注Supporting Products
Publication Part Number 5991-3600EN
Analysis Analysed with Part No.
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6550
Analytical Technique Technique
LC
UHPLC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
peptides
proteins
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Intact Protein Analysis Using an Agilent 6550 Q-TOF Mass Spectrometer (PDF)

Accurate mass intact protein analysis using 6550 iFunnel Q-TOF

  • Publication Part Number: 5991-2116EN
  • Created: 29 Mar 2013
  • 613 KB
  • PDF
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Publication Part Number 5991-2116EN
Analysis Analysed with Part No.
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6550
Analytical Technique Technique
LC
LC/MS
Injection
valve
Detection
LC/MS/MS
Sample Chemical Group
proteins
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Reversed-Phase Optimization for Ultra Fast Profiling of Intact and Reduced Monoclonal Antibodies using Agilent ZORBAX Rapid Resolution High Definition 300SB-C3 Column (PDF)

必威体育登录手机Rapid reversed-phase separations of intact and reduced monoclonal antibodies (mAbs) were optimized using Agilent ZORBAX RRHD 300SB-C3 columns.

  • Publication Part Number: 5990-9667EN
  • Created: 13 Feb 2012
  • 364 KB
  • PDF
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Publication Part Number 5990-9667EN
Analysis Analysed with Part No.
ZORBAX 300SB-C3
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Ultra High Speed and High Resolution Separations of Reduced and Intact Monoclonal Antibodies with Agilent ZORBAX RRHD Sub-2 μm 300 Diphenyl UHPLC Column (PDF)

JM

  • Publication Part Number: 5990-9668EN
  • Created: 07 Feb 2012
  • 807 KB
  • PDF
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Publication Part Number 5990-9668EN
Analysis Analysed with Part No.
ZORBAX 300-Diphenyl
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
示例矩陣
chinese hamster ovary
test mixture

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