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Separations of sugars using HPLC with Hi-Plex columns and water eluents are easily achieved, avoiding the need for toxic acetonitrile.
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The separation and detection of raffinose, lactose, glucose, galactose, and fructose are readily achieved using water as the mobile phase with a Hi-Plex Ca column and an ELSD.
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Combining the Hi-Plex Ca column with the ELSD provides an excellent solution for resolving saccharides which require sensitive and robust methods for their identification.
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