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Biopharma HPLC Analysis (8)
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Post Translational Modifications and Degradation (6)
Titer and Protein Quantitation (3)
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Mass Spectrometry of Proteins (1)
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LC (11)
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Bio-Monolith Protein A (3)
Bio Mab NP 5 (2)
Bio WCX NP5 (2)
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proteins (5)
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Example category image description
Applications
Combining small-scale purification and analysis of monoclonal antibodies on one instrument (PDF)

必威体育登录手机Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.

  • Publication Part Number: 5991-1195EN
  • Created: 01 May 2016
  • 854 KB
  • PDF
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Publication Part Number 5991-1195EN
Analysis Analysed with Part No.
Bio Mab
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
DAD
Sample Chemical Group
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Reducing Cycle Time for Quantification of Human IgG Using the Agilent Bio-Monolith Protein A HPLC Column (PDF)

必威体育登录手机This Application Note describes the quantification of human IgG with the Agilent Bio-Monolith Protein A HPLC Column with alternating column regeneration.

  • Publication Part Number: 5991-4723EN
  • Created: 01 June 2014
  • 836 KB
  • PDF
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Publication Part Number 5991-4723EN
Analysis Analysed with Part No.
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures (PDF)

In this application note, pre-packed Bio-monolith Protein A columns, part of the AdvanceBio family, were used to demonstrate the quick capture of monoclonal antibody titer

  • Publication Part Number: 5991-2990EN
  • Created: 08 Jan 2014
  • 441 KB
  • PDF
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Publication Part Number 5991-2990EN
Analysis Analysed with Part No.
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
示例矩陣
cell culture
Example category image description
Applications
必威体育登录手机Agilent Solutions for Removal and Monitoring of Genomic DNA from Monoclonal Antibody Preparations (PDF)

This Application Note describes an application solution for the removal and monitoring of genomic DNA contamination from monoclonal antibody preparation.

  • Publication Part Number: 5991-3527EN
  • Created: 01 Nov 2013
  • 987 KB
  • PDF
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Publication Part Number 5991-3527EN
Analysis Analysed with Part No.
Bio SEC-3
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
DNAs
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Optimizing Protein Separations with Agilent Weak Cation-Exchange Columns (PDF)

Columns containing weak cation-exchange stationary phases offer a degree of versability when used for separation of positively charged proteins.

  • Publication Part Number: 5990-9628EN
  • Created: 01 Aug 2013
  • 1 MB
  • PDF
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Publication Part Number 5990-9628EN
Analysis Analysed with Part No.
Bio WCX NP5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
proteins
示例矩陣
test mixture
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Faster Separations Using Agilent Weak Cation Exchange Columns (PDF)

Ion exchange is a commonly used technique for the separation of complex protein mixtures.

  • Publication Part Number: 5990-9931EN
  • Created: 01 Aug 2013
  • 2 MB
  • PDF
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Publication Part Number 5990-9931EN
Analysis Analysed with Part No.
Bio WCX NP1.7
Bio WCX NP3
Bio WCX NP5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
enzymes
nucleases
proteins
示例矩陣
test mixture
Example category image description
Applications
Analysis of proteins by anion exchange chromatography (PDF)

This shows the separation of four proteins by anion exchange chromatography using 4 different high salt-containing elution buffers (up to 2 M) for linear and step gradients.

  • Publication Part Number: 5990-9614EN
  • Created: 01 Aug 2013
  • 658 KB
  • PDF
Quick View Supporting Documents
Publication Part Number 5990-9614EN
Analysis Analysed with Part No.
---
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
proteins
示例矩陣
bovine milk
egg white
skeletal muscle
soybean
test mixture
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
Multiple Detector Approaches to Protein Aggregation by SEC (PDF)

必威体育登录手机Methods are given for the use of Agilent Bio-SEC columns, with LS, UV and RI detection, for quantification or quality control of protein aggregates

  • Publication Part Number: 5991-1400EN
  • Created: 07 Jan 2013
  • 284 KB
  • PDF
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Publication Part Number 5991-1400EN
Analysis Analysed with Part No.
Bio SEC-5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
UV
Sample Chemical Group
proteins
示例矩陣
test mixture
Example category image description
Applications
High-resolution Analysis of Charge Heterogeneity in Monoclonal Antibodies Using pH-gradient Cation Exchange Chromatography (PDF)

必威体育登录手机This Application Note describes a high-resolution, pH-based separation of acidic and basic charge variants for monoclonal antibodies using the Agilent 1260 and BiomAb PEEK

  • Publication Part Number: 5991-1407EN
  • Created: 01 Dec 2012
  • 805 KB
  • PDF
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Publication Part Number 5991-1407EN
Analysis Analysed with Part No.
Bio Mab NP 5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机利用 Agilent 1260 Infinity 生物惰性液相色譜係統及 Agilent ZORBAX RRHD 二苯基亞 2 μm 色譜柱分析 IgG1 中的二硫鍵 (PDF)

本應用簡報報導了一種簡易的反相 HPLC 方法,用於分析單克隆抗體 (mAb) 中的二硫鍵。

  • Publication Part Number: 5991-1694CHCN
  • Created: 01 Feb 2013
  • 1 MB
  • PDF
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Publication Part Number 5991-1694EN
Analysis Analysed with Part No.
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
pH 梯度洗脫用於改善單克隆抗體帶電變體的分離 (PDF)

pH 梯度洗脫用於改善單克隆抗體帶電變體的分離

  • Publication Part Number: 5990-9629CHCN
  • Created: 16 Dec 2011
  • 584 KB
  • PDF
Quick View 必威体育赛事投注Supporting Products
Publication Part Number 5990-9629EN
Analysis Analysed with Part No.
Bio Mab NP 5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
示例矩陣
test mixture

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