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Instructions for the QuikChange Lightning Site-Directed Mutagenesis Kit with detailed requirements, protocols, and transformation guides.
Materials, mutagenic primer design, mutant strand synthesis reaction, Dpn1 digestion, transformation of XL10-Gold Ultracompetent Cells.
Mutagenic primer design, mutant strand synthesis reaction, thermal cycling, Dpn I digestion, transformation of XL10-Gold Ultracompetent Cells, transformation guidelines.
Academic, QuikChange multi mutagenesis control, mutagenic primer design, mutant strand synthesis reaction, Dpn I digestion, transformation guidelines, and troubleshooting.
必威体育赛事投注Mutagenic primer design, mutant strand synthesis reaction, thermal cycling, Dpn I digestion of amplification products, transformation of XL1-Blue Supercompetent Cells.
必威体育赛事投注Materials, mutagenic primer design, mutant strand synthesis reaction, Dpn I digestion of amplification products, transformation of XL1-Blue Supercompetent Cells.
Commercial: QuikChange multi mutagenesis control, mutagenic primer design, mutant strand synthesis reaction, Dpn I digestion, transformation guidelines, and troubleshooting.
QuikChange II-E Site-Directed Mutagenesis Kit
Instruction manual for the commercial version of QuikChange Lightning Multi Site-Directed Mutagenesis Kit with introduction, guidelines and protocol requirements.
Instruction manual for the academic version of QuikChange Lightning Multi Site-Directed Mutagenesis Kit with introduction, guidelines and protocol requirements.
Mutational spectrum, amplification targets, initial amount of target DNA, cycle number, primer design, PCR product yield, achieving high mutation frequencies.