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Biopharma HPLC Analysis (7)
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Biopharma & Pharma Applications (6)
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Proteomics (6)
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LC (10)
LC/MS/MS (2)
LC/MS (1)
ZORBAX 300SB-C3 (2)
ZORBAX RRHD 300-DP (2)
ZORBAX 300SB-C18 (1)
ZORBAX 300SB-CN (1)
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monoclonal antibodies (8)
proteins (2)
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必威体育登录手机Agilent 1290 Infinity (4)
必威体育登录手机Agilent 1260 Infinity (4)
必威体育登录手机Agilent 6530 (2)
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必威体育登录手机Agilent 1200 (1)
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Applications
必威体育登录手机Analysis of Monoclonal Antibody (mAb) Using Agilent 1290 Infinity LC System Coupled to Agilent 6530 Accurate-Mass Quadrupole Time-of-Flight (Q-TOF) (PDF)

This work describes a high flow LCMS method to demonstrate the high resolution mass analysis of mAbs with good chromatography and its utility in analysis of Biosimilar mAbs.

  • Publication Part Number: 5991-4266EN
  • Created: 01 July 2014
  • 310 KB
  • PDF
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Publication Part Number 5991-4266EN
Analysis Analysed with Part No.
Poroshell 300SB-C8
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6530
Analytical Technique Technique
LC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Agilent Bio-Monolith Protein A Monitors Monoclonal Antibody Titer from Cell Cultures (PDF)

In this application note, pre-packed Bio-monolith Protein A columns, part of the AdvanceBio family, were used to demonstrate the quick capture of monoclonal antibody titer

  • Publication Part Number: 5991-2990EN
  • Created: 08 Jan 2014
  • 441 KB
  • PDF
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Publication Part Number 5991-2990EN
Analysis Analysed with Part No.
Bio-Monolith Protein A
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
示例矩陣
cell culture
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
Fast and Efficient Peptide Mapping of a Monoclonal Antibody (mAb): UHPLC Performance with Superficially Porous Particles (PDF)

必威体育登录手机The Agilent AdvanceBio Peptide Mapping column separates mAb tryptic digests at low system pressures, providing UHPLC performance from HPLC operating conditions.

  • Publication Part Number: 5991-3585EN
  • Created: 06 Dec 2013
  • 2 MB
  • PDF
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Publication Part Number 5991-3585EN
Analysis Analysed with Part No.
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6530
Analytical Technique Technique
LC
Injection
valve
Detection
MS-QTOF
Sample Chemical Group
monoclonal antibodies
peptides
示例矩陣
test mixture
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Agilent Solutions for Removal and Monitoring of Genomic DNA from Monoclonal Antibody Preparations (PDF)

This Application Note describes an application solution for the removal and monitoring of genomic DNA contamination from monoclonal antibody preparation.

  • Publication Part Number: 5991-3527EN
  • Created: 01 Nov 2013
  • 987 KB
  • PDF
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Publication Part Number 5991-3527EN
Analysis Analysed with Part No.
Bio SEC-3
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
DNAs
monoclonal antibodies
示例矩陣
test mixture
Example category image description
Applications
必威体育登录手机Analysis of Intact and C-terminal Digested IgG1 on an Agilent Bio MAb NP5 Column (PDF)

必威体育登录手机Agilent Bio MAb columns, in an Agilent 1260 Infinity Bio-inert Quaternary LC, characterize the charge heterogeneity of IgG1monoclonal antibody...

  • Publication Part Number: 5991-0895EN
  • Created: 01 Aug 2013
  • 240 KB
  • PDF
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Publication Part Number 5991-0895EN
Analysis Analysed with Part No.
Bio Mab NP 5
Instrument
必威体育登录手机Agilent 1100
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
bio-therapeutics
monoclonal antibodies
示例矩陣
chinese hamster
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
Characterization of Glycosylation in the Fc Region of Therapeutic Recombinant Monoclonal Antibody (PDF)

Fast and efficient LC TOF/MS characterizes intact and digested IgG to identify glycoform masses and profiles.mAb-glyco chip analysis built a glycan accurate mass database.

  • Publication Part Number: 5991-2323EN
  • Created: 23 Apr 2013
  • 475 KB
  • PDF
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Publication Part Number 5990-2323EN
Analysis Analysed with Part No.
Poroshell 300SB-C3
Instrument
必威体育登录手机Agilent 1200
Analytical Technique Technique
LC
LC/MS
LC/MS/MS
Injection
valve
Detection
DAD
LC/MS/MS
UV
Sample Chemical Group
antibodies
biotherapeutic drugs
pharmaceuticals
示例矩陣
chinese hamster ovary
Example category image description
Applications
必威体育登录手机Disulfide Linkage Analysis of IgG1 using an Agilent 1260 Infinity Bio-inert LC System with an Agilent ZORBAX RRHD Diphenyl sub-2 μm Column (PDF)

This Application Note describes a simple method for the analysis of disulfide linkages in monoclonal antibodies (mAbs) by reversed phase HPLC.

  • Publication Part Number: 5991-1694EN
  • Created: 01 Feb 2013
  • 899 KB
  • PDF
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Publication Part Number 5991-1694EN
Analysis Analysed with Part No.
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
DAD
Sample Chemical Group
monoclonal antibodies
示例矩陣
test mixture
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Reversed-Phase Optimization for Ultra Fast Profiling of Intact and Reduced Monoclonal Antibodies using Agilent ZORBAX Rapid Resolution High Definition 300SB-C3 Column (PDF)

必威体育登录手机Rapid reversed-phase separations of intact and reduced monoclonal antibodies (mAbs) were optimized using Agilent ZORBAX RRHD 300SB-C3 columns.

  • Publication Part Number: 5990-9667EN
  • Created: 13 Feb 2012
  • 364 KB
  • PDF
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Publication Part Number 5990-9667EN
Analysis Analysed with Part No.
ZORBAX 300SB-C3
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
示例矩陣
test mixture
Japanese (Japan) Complete (PDF)
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Ultra High Speed and High Resolution Separations of Reduced and Intact Monoclonal Antibodies with Agilent ZORBAX RRHD Sub-2 μm 300 Diphenyl UHPLC Column (PDF)

JM

  • Publication Part Number: 5990-9668EN
  • Created: 07 Feb 2012
  • 807 KB
  • PDF
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Publication Part Number 5990-9668EN
Analysis Analysed with Part No.
ZORBAX 300-Diphenyl
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
示例矩陣
chinese hamster ovary
test mixture
Japanese (Japan) Complete (PDF)
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
Comparison of ZORBAX StableBond 300 Å LC Columns to Optimize Selectivity for Antibody Separations Using HPLC and LC/MS (PDF)

StableBond 300 Å Rapid Resolution (3.5 μm) LC columns with different bonded phases were used to systematically develop a reversed-phase LC/MS method for reduced...

  • Publication Part Number: 5989-6840EN
  • Created: 27 June 2007
  • 203 KB
  • PDF
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Publication Part Number 5989-6840EN
Analysis Analysed with Part No.
ZORBAX 300SB-C18
ZORBAX 300SB-C3
ZORBAX 300SB-C8
ZORBAX 300SB-CN
Instrument
---
Analytical Technique Technique
LC
LC/MS/MS
Injection
valve
Detection
LC/MS/MS
UV
Sample Chemical Group
antibodies
glycoproteins
示例矩陣
pharmaceuticals
Japanese (Japan) Complete (PDF)

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