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Biopharma HPLC Analysis (5)
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LC (8)
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proteins (8)
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Applications
必威体育登录手机Determination of Protein Molecular Weight and Size Using the Agilent 1260 Infinity Multi-Detector Bio-SEC Solution with Advanced Light Scattering Detection (PDF)

必威体育登录手机This Application Note shows the determination of protein molecular weightand size, together with linear quantification using the Agilent 1260 Infinity Multi-Detector Bio-SEC S

  • Publication Part Number: 5991-3955EN
  • Created: 01 Mar 2014
  • 957 KB
  • PDF
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Publication Part Number 5991-3955EN
Analysis Analysed with Part No.
Bio SEC-3
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
SEC
Injection
valve
Detection
DAD
LSD
UV
Sample Chemical Group
proteins
Sample Matrix
test mixture
Russian (Russia) Complete (PDF)
Example category image description
Applications
必威体育登录手机Faster Separations Using Agilent Weak Cation Exchange Columns (PDF)

Ion exchange is a commonly used technique for the separation of complex protein mixtures.

  • Publication Part Number: 5990-9931EN
  • Created: 01 Aug 2013
  • 2 MB
  • PDF
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Publication Part Number 5990-9931EN
Analysis Analysed with Part No.
Bio WCX NP1.7
Bio WCX NP3
Bio WCX NP5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
enzymes
nucleases
proteins
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Optimizing Protein Separations with Agilent Weak Cation-Exchange Columns (PDF)

Columns containing weak cation-exchange stationary phases offer a degree of versability when used for separation of positively charged proteins.

  • Publication Part Number: 5990-9628EN
  • Created: 01 Aug 2013
  • 1 MB
  • PDF
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Publication Part Number 5990-9628EN
Analysis Analysed with Part No.
Bio WCX NP5
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
proteins
Sample Matrix
test mixture
Japanese (Japan) Complete (PDF)
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Choosing the Right Calibration for the Agilent Bio SEC-3 Column (PDF)

Column choice in SEC depends on column resolving range, identified by calibration with standards appropriate to analyte type, molecular weight range, and size in solution.

  • Publication Part Number: 5991-2463EN
  • Created: 14 May 2013
  • 117 KB
  • PDF
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Publication Part Number 5991-2463EN
Analysis Analysed with Part No.
Bio SEC-3 300A
Instrument
必威体育登录手机Agilent 1260 Infinity
Analytical Technique Technique
GPC
LC
SEC
Injection
valve
Detection
RI
UV
Sample Chemical Group
polymers
polysaccharides
proteins
Sample Matrix
test mixture
Example category image description
Applications
Choosing the Best Filters for Biological Samples Filtration (PDF)

必威体育登录手机Agilent Captiva Premium PES syringe filters are compared with non-Agilent PES and PVDF syringe filters.

  • Publication Part Number: 5991-1308EN
  • Created: 26 Oct 2012
  • 293 KB
  • PDF
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Publication Part Number 5991-1308EN
Analysis Analysed with Part No.
Bio SEC-3 300A
Captiva premium PES
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6150 Single Quadrupole LC/MS
Analytical Technique Technique
Filtration
LC
LC/MS
SPE
Injection
valve
Detection
LC/MS/MS
UV
Sample Chemical Group
proteins
Sample Matrix
bovine heart
bovine serum
bovine thyroid
chicken egg
horse heart
Example category image description
Applications
必威体育登录手机Reversed-Phase Optimization for Ultra Fast Profiling of Intact and Reduced Monoclonal Antibodies using Agilent ZORBAX Rapid Resolution High Definition 300SB-C3 Column (PDF)

必威体育登录手机Rapid reversed-phase separations of intact and reduced monoclonal antibodies (mAbs) were optimized using Agilent ZORBAX RRHD 300SB-C3 columns.

  • Publication Part Number: 5990-9667EN
  • Created: 13 Feb 2012
  • 364 KB
  • PDF
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Publication Part Number 5990-9667EN
Analysis Analysed with Part No.
ZORBAX 300SB-C3
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
Sample Matrix
test mixture
Japanese (Japan) Complete (PDF)
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Ultra High Speed and High Resolution Separations of Reduced and Intact Monoclonal Antibodies with Agilent ZORBAX RRHD Sub-2 μm 300 Diphenyl UHPLC Column (PDF)

JM

  • Publication Part Number: 5990-9668EN
  • Created: 07 Feb 2012
  • 807 KB
  • PDF
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Publication Part Number 5990-9668EN
Analysis Analysed with Part No.
ZORBAX 300-Diphenyl
ZORBAX RRHD 300-DP
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
monoclonal antibodies
proteins
Sample Matrix
chinese hamster ovary
test mixture
Japanese (Japan) Complete (PDF)
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
必威体育登录手机Fast Protein Separations Using Agilent Poroshell 300 (PDF)

The number of proteins and antibodies in drug development and in clinical phase trials is growing rapidly.

  • Publication Part Number: 5989-9899EN
  • Created: 25 Nov 2008
  • 110 KB
  • PDF
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Publication Part Number 5989-9899EN
Analysis Analysed with Part No.
Poroshell 300Extend-C18
Poroshell 300SB-C18
Poroshell 300SB-C3
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
antibodies
biomolecules
peptides
proteins
Sample Matrix
clinical samples
drug samples

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