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Applications
必威体育登录手机Automation for LC/MS Sample Preparation: High Throughput In-Solution Digestion and Peptide Cleanup Enabled by the Agilent AssayMAP Bravo Platform (PDF)

Protein and peptide sample preparation for LC/MS remains entrenched in workflows reliant upon serial manual manipulations.

  • Publication Part Number: 5991-2957EN
  • Created: 16 Mar 2016
  • 1 MB
  • PDF
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Publication Part Number 5991-2957EN
Analysis Analysed with Part No.
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6550
Analytical Technique Technique
LC
LC/MS
Injection
valve
Detection
LC/MS/MS
Sample Chemical Group
peptides
proteins
Sample Matrix
bovine serum albumin
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
Workflow Automation for LC/MS: In-Solution Protein Digestion, Peptide Cleanup, and Strong Cation-Exchange Fractionation of Peptides Enabled by AssayMAP Technology (PDF)

Proof of principal, discovery-style experiment demonstrating high precision & ease of use for complex multistep peptide sample preparation workflow automated on AssayMAP tech.

  • Publication Part Number: 5991-3602EN
  • Created: 16 Mar 2016
  • 5 MB
  • PDF
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Publication Part Number 5991-3602EN
Analysis Analysed with Part No.
93 C18 cartridge rack
96 C18 cartridges and labware for digestion and cleanup
96 RP-S cartridge rack
96 RP-S cartridges and labware for digestion and cleanup
96 SCX cartridge rack
96 SCX cartridges and labware for fractionation
96 resin-free cartridge rack
AdvanceBio Peptide Map
Instrument
必威体育登录手机Agilent 1290 Infinity
必威体育登录手机Agilent 6550
Analytical Technique Technique
LC
SPE
Injection
valve
Detection
MS
Sample Chemical Group
peptides
proteins
Sample Matrix
ecoli tryptic digest
test mixture
Chinese (Simplified) (China) Complete (PDF)
Example category image description
Applications
CE/MS/MS as an Orthogonal Technique for Sensitive and Easy Quantification of Peptides in Complex Matrixes (PDF)

This Application Note describes an orthogonal approach to liquid chromatography/ mass spectrometry (LC/MS)

  • Publication Part Number: 5991-2729EN
  • Created: 01 Apr 2014
  • 1 MB
  • PDF
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Publication Part Number 5991-2729EN
Analysis Analysed with Part No.
---
Instrument
必威体育登录手机Agilent 6490 Triple Quadrupole Mass spectrometer
必威体育登录手机Agilent 7100 capillary electrophoresis system
Analytical Technique Technique
Electrophoresis
Injection
---
Detection
MS
Sample Chemical Group
peptides
Sample Matrix
test mixture
Example category image description
Applications
Peptides, amidated and deamidated (PDF)

必威体育登录手机HPLC using an Agilent Pursuit C18 column separates amidated and deamidated peptides in under six minutes.

  • Publication Part Number: A02298
  • Created: 31 Oct 2011
  • 478 KB
  • PDF
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Publication Part Number A02298
Analysis Analysed with Part No.
Pursuit C18
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
peptides
Sample Matrix
test mixture
Example category image description
Applications
必威体育登录手机Analyze MAb and BSA digests by UHPLC with UV detection and Agilent ZORBAX RRHD 300SB-C18 (PDF)

必威体育登录手机Application note for trypsin-digested monoclonal antibody and BSA using Agilent ZORBAX RRHD 300SB-C18

  • Publication Part Number: 5990-8244EN
  • Created: 01 June 2011
  • 4 MB
  • PDF
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Publication Part Number 5990-8244EN
Analysis Analysed with Part No.
ZORBAX RRHD 300SB-C18
Instrument
必威体育登录手机Agilent 1290 Infinity
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
antibodies
peptides
proteins
Sample Matrix
test mixture
Example category image description
Applications
Investigation into the Alternatives to Acetonitrile for the Analysis of Peptides on a VariTide RPC (PDF)

Application note for the investigation into the Alternatives to Acetonitrile for the Analysis of Peptides on a VariTide RPC

  • Publication Part Number: 5990-8145EN
  • Created: 19 May 2011
  • 664 KB
  • PDF
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Publication Part Number 5990-8145EN
Analysis Analysed with Part No.
VariTide RPC
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
peptides
proteins
Sample Matrix
test mixture
Example category image description
Applications
Gradient Purification of Synthetic Acyl Carrier Protein Fragment 65-74 (PDF)

Application note for the Gradient Purification of Synthetic Acyl Carrier Protein Fragment 65-74

  • Publication Part Number: 5990-7738EN
  • Created: 11 Apr 2011
  • 572 KB
  • PDF
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Publication Part Number 5990-7738EN
Analysis Analysed with Part No.
PLRP-S 100Å
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
acyl carrier proteins
peptides
Sample Matrix
test mixture
Example category image description
Applications
Isocratic Purification of Synthetic Acyl Carrier Protein Fragment 65-74 (PDF)

Application note for the isocratic purification of Synthetic Acyl Carrier Protein Fragment 65-74

  • Publication Part Number: 5990-7737EN
  • Created: 11 Apr 2011
  • 669 KB
  • PDF
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Publication Part Number 5990-7737EN
Analysis Analysed with Part No.
PLRP-S 100Å
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
biomolecules
oligonucleotides
peptides
Sample Matrix
test mixture
Example category image description
Applications
Peptides (PDF)

必威体育登录手机Separating five peptides by HPLC in 12 minutes is straightforward with Agilent Pursuit C8 5 µm material in a ChromSep cartridge.

  • Publication Part Number: A2330
  • Created: 11 May 2010
  • 554 KB
  • PDF
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Publication Part Number A02330
Analysis Analysed with Part No.
Pursuit C8
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
peptides
Sample Matrix
test mixture
Example category image description
Applications
Fast Separation of Large and Heterogeneous Proteins Using ZORBAX Poroshell C18, C8, and C3 Phases.(PDF)

The use of C18-bonded totally porous particle columns is the traditional way to separate proteins by reversed phase liquid chromatography.

  • Publication Part Number: 5989-0015EN
  • Created: 17 Oct 2003
  • 640 KB
  • PDF
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Publication Part Number 5989-0015EN
Analysis Analysed with Part No.
Poroshell 300SB-C18
Poroshell 300SB-C3
Poroshell 300SB-C8
ZORBAX 300SB-C18
Instrument
---
Analytical Technique Technique
LC
Injection
valve
Detection
UV
Sample Chemical Group
peptides
proteins
Sample Matrix
test mixture

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