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Columns containing weak cation-exchange stationary phases offer a degree of versability when used for separation of positively charged proteins.
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Ion exchange is a commonly used technique for the separation of complex protein mixtures.
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Ion exchange is a very useful technique to separate protein mixtures under mild conditions, most commonly by applying a shallow gradient of increasing salt concentration as el
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This shows the separation of four proteins by anion exchange chromatography using 4 different high salt-containing elution buffers (up to 2 M) for linear and step gradients.
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