Note: Preparative to analytical scaling calculations do not take dwell volume into consideration.
Analytical Method | Preparative Method | |||
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System Description | ||||
Name of each chromatographic instrument (e.g., "1260 Infinity II Preparative LC" or "1260 Infinity II Analytical LC") |
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Minimum instrument flow rate |
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Maximum instrument flow rate |
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Volume from the mobile phase mixing point to the column inlet.Learn more |
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Time required for the autosampler to complete one injection cycle |
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Solvent name (e.g., "Water + 0.1% formic acid") |
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Solvent name (e.g., "Acetonitrile + 0.1% formic acid") |
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Column Information | ||||
Stationary phase name (e.g., "ZORBAX SB-C18"). |
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Column particle size |
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Column internal diameter |
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Column length |
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Method flow rate |
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This value exceeds the maximum flow rate.The gradient table will be calculated using the maximum flow rate. | ||||
Method injection volume |
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Estimated concentration of the target compound in the sample |
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Override preparative flow rate
Select "yes" to scale the analytical gradient program to a user-defined preparative flow rate!select "no" to use the preparative flow rate scaled from the analytical flow rate |
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Total mass of purified target compound |
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Current mass on column (mg) | ||||
Estimated column capacity (mg) | ||||
Column void volume (mL) |
Analytical Gradient | Preparative Gradient | ||||||
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Time (min) | Flow (mL/min) | %A | %B | Time (min) | Flow (mL/min) | %A | %B |
Use the following step gradient method to determine the dwell volume of a chromatographic system.
Note that when using sample loops with volumes larger than 5 mL, the flow rate needs to be increased.A good compromise is to keep the flow rate equal to the loop size.