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Peptide Separations Reversed-Phase Bio Columns (43)

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ZORBAX 300StableBond HPLC Columns

For reversed-phase selectivity optimization of peptides and proteins, available in four different bonded phases.

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ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons.Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase.Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases.For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides.To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C.Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.

ZORBAX GF-250/450 SEC Columns

Legacy zirconium-stabilized silica diol size exclusion columns (USP designation L35).

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必威体育登录手机Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules.The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series.|The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8.The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.|The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min.These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.|Common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins.

ZORBAX 300Extend-C18

ZORBAX 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH.

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ZORBAX 300Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2-11.5.Extend-C18 and 300Extend-C18 columns incorporate a unique bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH.At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge.Excellent recoveries of hydrophobic polypeptides have been achieved at high pH with the 300Extend C18.In addition, LC/MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase.These conditions allow for ions to be detected in both positive and negative ion modes simultaneously with the 300Extend-C18.The ZORBAX 300Extend-C18 is a rugged column and is ideal for LC/MS with ammonium hydroxide mobile phase.View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.

ZORBAX Ion Exchange Columns

ZORBAX strong anion exchange (SAX) and strong cation exchange (300SCX) columns provide optimum efficiency for separation and purifying biological molecules.

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ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns.Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides and polynucleotides.ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability.Therefore, they can be used at high operating flow rates and pressures.The columns are compatible with rapid changes in ionic strength, resulting in short gradient times and rapid re-equilibration are possible.

ZORBAX RRHD Wide Pore Reversed Phase HPLC Columns

Ideal for reproducible separations of proteins and peptides!available in four different bonded phases: C18, C8, C3, and Diphenyl.

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ZORBAX 300Å StableBond columns are ideal for the reproducible separations of proteins and peptides.The wide, 300Å pore on these reversed-phase HPLC columns is essential for an efficient separation of these large biomolecules, in order for them to completely access the bonded phase.The ZORBAX RRHD columns have 1.8 µm particles which are compatible with UHPLC and ideal for higher resolution separations with shorter run times.StableBond columns are available in four different bonded phases (C18, C8, C3, and Diphenyl) for selectivity and recovery optimization of proteins and polypeptides.To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80°C.300SB-C18 and 300SB-C8 columns are an ideal choice for complex protein and protein digest separations.ZORBAX RRHD 300-HILIC columns are also available for glycopeptide analysis, which enable you to resolve peptide glycoforms.

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5064-8259

ZORBAX 300SB-C18 3.5µm, 0.3 x 100 mm.A reversed-phase C18 HPLC column with fully porous ZORBAX particles.StableBond technology ensures stability at low pH.

Unit:
1 Each
Length:
100 mm

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