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Cloning Kits

必威体育登录手机Agilent offers everything for difficult cloning projects with a traditional topoisomerase-based kit to a huge selection of catalog vectors for all PCR applications.

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Product Collections
PCR-Based Detection Kits

Detect mycoplasma infections of cell cultures or directly amplify your DNA sample from liquid or dried blood.

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Cloning & PCR Detection Kits

A wide variety of PCR polymerases and reagents, PCR-based specialty kits and reagents, PCR inhibitors, and cloning kits to serve your PCR DNA amplification needs.

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240205

StrataClone PCR Cloning Kit.High efficiency UA PCR cloning featuring DNA topoisomerase I and Cre recombinase technologies.Sufficient for 20 reactions.

  • Regulatory Status:

    RUO

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G7516B

SureVector Yeast Mammalian C-terminal Expansion Kit.Promoters and C-terminal tags for expression in mammalian cells.

  • Regulatory Status:

    RUO

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240218

StrataClone Ultra Blunt PCR Cloning Kit.High efficiency blunt-end PCR cloning, features DNA topoisomerase I and Cre recombinase technologies.Kit includes PfuUltra II for high fidelity PCR amplification.Sufficient for 20 reactions.

  • Regulatory Status:

    RUO

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G7514A

SureVector Core Kit.Contains the basic elements needed for functionality in bacterial, yeast, and mammalian systems.

  • Regulatory Status:

    RUO

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600930

SureDirect Blood PCR Kit.Specially formulated with a mutant of Taq DNA polymerase designed to overcome blood inhibitors for more robust performance.Designed for amplification of targets directly from whole blood and blood derivative samples.The 2X...

  • Regulatory Status:

    RUO

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G7516A

SureVector Mammalian N-terminal Expansion Kit.Promoters and N-terminal tags for expression in mammalian cells.

  • Regulatory Status:

    RUO

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240207

StrataClone Blunt PCR Cloning Kit.High efficiency blunt-end PCR cloning, features DNA topoisomerase I and Cre recombinase technologies.Sufficient for 20 reactions.

  • Regulatory Status:

    RUO

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600640

2x master mix format of Easy-A high-fidelity PCR cloning enzyme

  • Regulatory Status:

    RUO
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600404

Easy-A high-fidelity PCR cloning enzyme offers high throughput cloning into T/U-modified vectors without having to perform an \'A-addition\' step.

  • Volume:

    5 U/µL

  • Regulatory Status:

    RUO
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PCR Polishing Kit

PCR polishing kit increases blunt-end cloning efficiencies associated with PCR-generated fragments.

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PCR Polishing Kit enables an increase in blunt-ended cloning efficiencies associated with polymerase chain reaction (PCR)-generated fragments.It polishes the ends of the 3' overhang extensions of polymerase-generated DNA fragments directly from PCR amplification and can completely fill in 5' overhangs to generate blunt ends.必威体育登录手机|Dramatic increases in the population of blunt-end DNA fragments following polishing with Agilent's PCR Polishing Kit enables significant increase in overall experimental efficiency associated with procedures utilizing blunt-ended ligation.
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StrataClone PCR Cloning Kits

Fast and effective room temperature PCR cloning in just 5 minutes.

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必威体育登录手机The Agilent StrataClone PCR cloning kit enables high-efficiency, 5-minute cloning of PCR products at room temperature.The kits use the DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase to yield >95% insert positive clones.Available for both blunt-end and UA cloning, the kits can clone both long and short amplicons.|In addition, our StrataClone Ultra PCR Cloning System is also available.This kit includes the PfuUltra II Fusion Hotstart DNA Polymerase.The StrataClone Ultra system combines the highest accuracy in PCR amplification with easy, reliable, and affordably priced topoisomerase-based cloning.
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MycoSensor Assay Kits

Detect Mycoplasma infection of cell cultures by PCR in less than two hours.

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The MycoSensor PCR Assay Kits detect Mycoplasma infection of cell cultures by PCR in less than two hours.
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SureDirect Blood PCR Kit

Enables direct DNA amplification from liquid blood, dried blood on paper, serum, plasma, and more.

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The SureDirect Blood PCR Kit enables direct DNA amplification from many sample types including: liquid blood, dried blood on paper, serum, and plasma.Inhibitor resistance and throughput, common PCR challenges associated with blood samples, are averted through the activity of a newly engineered Taq.|Your workflow is streamlined by eliminating the cumbersome DNA extraction step.SureDirect Blood PCR Kit's outstanding robust performance will enable you to multiplex up to 3 targets and amplify high GC content (77%) and long amplicons (2.6 KB).
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SureVector

必威体育登录手机Combine standard DNA components to build custom vector constructs from Agilent's set of standard parts.

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必威体育登录手机SureVector next-generation cloning uses the power of synthetic biology to combine standard DNA components to build custom vector constructs from the Agilent set of standard parts.Simply design a vector, order or PCR your gene-of-interest, and your custom plasmid is just a single reaction away.|Proprietary enzymes enable rapid and reliable assembly of multiple DNA fragments into a circular, recombinant plasmid containing your target gene.Components include: bacterial selection markers, bacterial origins of replication, mammalian selection markers, promoters for yeast, mammalian cells and bacteria, and a variety of solubility/affinity tags|Enabling functionality in a range of cells.
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Easy-A Hi-Fi Cloning Enzyme

Combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq.

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必威体育登录手机The Agilent Easy-A high-fidelity PCR cloning enzyme is a proprietary thermostable DNA polymerase formulation specifically designed for improved cloning with the StrataClone PCR Cloning Kit or with the TOPO TA Cloning vector and other T-/U-vectors.|Easy-A combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq and increased specificity with added hot start antibody formulation.必威体育赛事投注PCR products amplified with Easy-A can be cloned directly, without performing the additional steps typically required when amplifying with proofreading polymerases.
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Brochures
Molecular and Synthetic Biology Solutions (PDF)

Reliable next-generation mutagenesis and cloning tools

  • Publication Part Number: 5991-9163EN
  • Created: 22 June 2023
  • 3 MB
  • PDF
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Catalogs
PCR Amplification (PDF)

PCR amplification reagents, instruments, and consumables catalog

  • Publication Part Number: 5991-9020EN
  • Created: 28 Feb 2022
  • 3 MB
  • PDF
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