QuikChange Primer Design

The QuikChange® Primer Design Program supports mutagenic primer design for your QuikChange mutagenesis experiments.Using primer design guidelines described in QuikChange manuals, this program calculates/designs the appropriate primer sequences with the optimal melting temperature.Read Helpfor more information about the program.
1.It is recommended that you clear this form prior to loading each new sequence:
2.Select QuikChange® mutagenesis kit that you are using:
3.Find your DNA sequence by pressing
Load a file from your hard-drive:
Or, paste plain text or FASTA-formatted DNA sequence in the box below:
4.Load it.
- or -
or specify a DNA region to translate from to
Finally, obtain your designed primer sequences.
Clear this form to load a new sequence:
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