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DNase I, RNase Free

Part Number:
600032
RUO

CRNase Free DNase I.contains mixture of four isoenzymes from bovine pancreas (10 units/µL)

For Research Use Only.Not for use in diagnostic procedures.
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Specifications
Concentration
  • 10 U/µL
Incubation Time
  • Incubate 30 minutes at 37 °C
Physical Form
  • Premixed
Reaction Conditions
  • 40 mM Tris-HCl (pH 7.5), 6 mM MgCl2, 2 mM CaCl2, 1 µg DNA and 1 unit enzyme in a 25 µL volume.Incubate 30 minutes at 37°C.
Reactions per Kit
  • 5000 units
Storage Buffer
  • 10mM Tris-HCl (pH 7.5), 10 mM CaCl2, 10 mM MgCl2 and 50 % glycerol (v/v) (Dilutions should be made in this buffer.)
Substrate Type
  • RNA
Unit Definition
  • One unit will completely digest 1 µg of DNA in 10 minutes at 37°C in a 25 µL reaction volume.
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