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ZORBAX 300StableBond HPLC Columns

For reversed-phase selectivity optimization of peptides and proteins, available in four different bonded phases.

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ZORBAX 300Å StableBond columns are an ideal choice for the reproducible separations of proteins and peptides for two reasons.Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase.Second, 300StableBond columns are unmatched in their durability at low pH, such as with TFA-containing mobile phases.For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.These columns are available in five different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides.To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80 °C.Capillary and nano columns can be used for either 1-D or 2-D proteomics separations.View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.
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ZORBAX GF-250/450 SEC Columns

Legacy zirconium-stabilized silica diol size exclusion columns (USP designation L35).

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&#24517;&#23041;&#20307;&#32946;&#30331;&#24405;&#25163;&#26426;Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules.The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series.|The GF-250/GF-450 size exclusion columns have a hydrophilic diol bonded phase for high recovery of proteins (typically >90%) and a unique zirconia modification of the silica for a pH operating range from 3-8.The GF-250 and GF-450 columns are packed with precisely sized porous silica microspheres with narrow pore size and particle size distributions.|The result is a column that can be used for both analytical and preparative separations of proteins with flow rates of up to 3 mL/min.These columns are compatible with organic modifiers (<25%) and denaturants in the mobile phase to reduce protein aggregation.|Common applications include separations of protein monomers, dimers and aggregates, desalting, protein molecular weight estimation and separations of modified proteins.
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ZORBAX Reversed-Phase HPLC Columns

Proven, fully porous column technology based on ZORBAX silica for reversed-phase HPLC separations.

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ZORBAX is one of the most popular high-performance reversed-phase HPLC column families.ZORBAX columns are based on traditional fully porous particles and offer the highest loading capacity and resolution.From Eclipse Plus, Eclipse XDB, StableBond, Bonus-RP and more, the ZORBAX column portfolio offers a wide selection of stationary phases allowing you to fine-tune your selectivity to match your application.Improve resolution by utilizing sub-2 µm particles to maximize system efficiency and confidently scale-up to preparative HPLC and/or easy HPLC method transfer.
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ZORBAX 300Extend-C18

ZORBAX 300Extend-C18 columns incorporate a unique patented bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH.

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ZORBAX 300Extend-C18 is a wide-pore HPLC column for high efficiency separations of peptides, polypeptides, and small proteins from pH 2-11.5.Extend-C18 and 300Extend-C18 columns incorporate a unique bidentate silane, combined with a double-endcapping process that protects the silica from dissolution at high pH.At high pH, the retention and selectivity of peptides can change dramatically as a result of changes in amino acid charge.Excellent recoveries of hydrophobic polypeptides have been achieved at high pH with the 300Extend C18.In addition, LC/MS sensitivity of peptides can also be improved at high pH using a simple ammonium hydroxide mobile phase.These conditions allow for ions to be detected in both positive and negative ion modes simultaneously with the 300Extend-C18.The ZORBAX 300Extend-C18 is a rugged column and is ideal for LC/MS with ammonium hydroxide mobile phase.View our full range of bioHPLC columns for your area of interest, get resources, and take advantage of special offers.
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ZORBAX Ion Exchange Columns

ZORBAX strong anion exchange (SAX) and strong cation exchange (300SCX) columns provide optimum efficiency for separation and purifying biological molecules.

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ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns.Each column is packed with bonded, 5 µm, spherical silica particles for optimum efficiency in separating and purifying biological macromolecules, including proteins, peptides and polynucleotides.ZORBAX Ion Exchange columns exhibit a high degree of mechanical stability.Therefore, they can be used at high operating flow rates and pressures.The columns are compatible with rapid changes in ionic strength, resulting in short gradient times and rapid re-equilibration are possible.
Community Search Results

以下是隨意重寫的 "Zorbax"：RRHD SB-C8 Carry Over of Domoic Acid

以下是隨意重寫的 "Zorbax"：RRHD SB-C8 Carry Over of Domoic Acid

Hello Everyone, I am currently trying to separate Domoic Acid in Mussel Tissue on a以下是隨意重寫的 "Zorbax"：SB-C8 column using a 1290 UHPLC and 6470 Triple Quad MS, the issue I am seeing as that the Domoic Acid is sitting in the column as no injections and MeOH solvent injections contain the peak in question for Domoic Acid.

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Guard Column for我無法重寫沒有提供具體內容的文本。Eclipse Plus C18 4.6X100mm 3.5um

Guard Column for我無法重寫沒有提供具體內容的文本。Eclipse Plus C18 4.6X100mm 3.5um

&#24517;&#23041;&#20307;&#32946;&#30331;&#24405;&#25163;&#26426;I have been browsing the agilent website and the column compatibility guide and I'm getting some conflicting information on what is the correct guard cartridge and holder for this column.For reference the part number is 959961-902.Thanks in advance!

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Guard Column for a我無法重寫沒有提供具體內容的文本。SB-C18, 2.1 x 150 mm, 5 µm LC Column

Guard Column for a我無法重寫沒有提供具體內容的文本。SB-C18, 2.1 x 150 mm, 5 µm LC Column

I would like to buy a guard column for a我無法重寫沒有提供具體內容的文本。&#24517;&#23041;&#20307;&#32946;&#30331;&#24405;&#25163;&#26426;SB-C18, 2.1 x 150 mm, 5 µm LC column but I´m having a hard time in the Agilent website.Which part number is the correct one?

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以下是隨意重寫的 "Zorbax"：Eclipse AAA column which USP packing material (e.g.L1/L7/L3), is it stored?

以下是隨意重寫的 "Zorbax"：Eclipse AAA column which USP packing material (e.g.L1/L7/L3), is it stored?

Please give me answer以下是隨意重寫的 "Zorbax"：Eclipse AAA column which USP column packing material is remained.e.g.L1.

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Example category image description
Zorbax HILIC

Traditional fully porous columns for HILIC separations of small, polar analytes.

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&#24517;&#23041;&#20307;&#32946;&#30331;&#24405;&#25163;&#26426;Agilent ZORBAX HILIC columns are designed for hydrophilic interaction chromatography (HILIC) applications and are typically used for the retention and resolution of small polar compounds.These columns are a traditional, fully porous alternative for your HILIC method development.The robustness and reproducibility of HILIC separations on ZORBAX columns help to switch from challenging, often MS-incompatible ion pair chromatography and normal phase applications.
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Zorbax Normal Phase SFC Columns

Fully porous columns based on ZORBAX silica for normal phase LC and SFC separations.

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ZORBAX fully porous particle columns for normal phase HPLC and supercritical fluid chromatography (SFC) are designed for the retention and separation of lipophilic compounds.The unique ZORBAX chemistries deliver outstanding efficiency in both achiral SFC and normal phase chromatography due to the similar retention mechanisms of both LC modes on the polar stationary phases used.The recommended ZORBAX phases are Rx-Sil, amino (NH 2), cyano (CN), Eclipse XDB-CN, and HILIC Plus (HILIC phases in SFC only).
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ZORBAX RRHD Wide Pore Reversed Phase HPLC Columns

Ideal for reproducible separations of proteins and peptides!available in four different bonded phases: C18, C8, C3, and Diphenyl.

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ZORBAX 300Å StableBond columns are ideal for the reproducible separations of proteins and peptides.The wide, 300Å pore on these reversed-phase HPLC columns is essential for an efficient separation of these large biomolecules, in order for them to completely access the bonded phase.The ZORBAX RRHD columns have 1.8 µm particles which are compatible with UHPLC and ideal for higher resolution separations with shorter run times.StableBond columns are available in four different bonded phases (C18, C8, C3, and Diphenyl) for selectivity and recovery optimization of proteins and polypeptides.To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80°C.300SB-C18 and 300SB-C8 columns are an ideal choice for complex protein and protein digest separations.ZORBAX RRHD 300-HILIC columns are also available for glycopeptide analysis, which enable you to resolve peptide glycoforms.

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5064-8253

ZORBAX StableBond C18, 0.3 x 35 mm, 5 µm, Cap/Nano Guard, 1/pk.Protects columns from particulates without compromising performance.Extends column life and maintain system efficiency.

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5064-8254

ZORBAX StableBond C18, 0.5 x 35 mm, 5 µm, Cap/Nano Guard, 1/pk.Protects columns from particulates without compromising performance.Extends column life and maintain system efficiency.

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5064-8255

ZORBAX StableBond C18 Column, 0.3 x 150 mm, 5 µm.Non-endcapped C18 phase with bulky diisobutyl side chains, offers stability for low pH down to pH 1, resisting degradation from aggressive mobile phases.Ideal for acidic conditions.

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5064-8256

ZORBAX StableBond C18 Column, 0.5 x 150 mm, 5 µm.Non-endcapped C18 phase with bulky diisobutyl side chains, offers stability for low pH down to pH 1, resisting degradation from aggressive mobile phases.Ideal for acidic conditions.

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5064-8259

ZORBAX 300SB-C18 3.5µm, 0.3 x 100 mm.A reversed-phase C18 HPLC column with fully porous ZORBAX particles.StableBond technology ensures stability at low pH.

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    100 mm

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5064-8260

ZORBAX RR StableBond C18 Column, 0.5 x 35 mm, 3.5 µm.Ideal for polar compound separations.Excellent peak shape and reproducibility.pH range: 2-9.

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5064-8261

ZORBAX RR StableBond C18 Column, 0.3 x 150 mm, 3.5 µm.Ideal for polar compound separations.Excellent peak shape and reproducibility.pH range: 2-9.

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    150 mm

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5064-8262

ZORBAX RR StableBond C18 Column, 0.5 x 150 mm, 3.5 µm.Ideal for polar compound separations.Excellent peak shape and reproducibility.pH range: 2-9.

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    150 mm

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5064-8263

ZORBAX 300SB-C18 5µm, 0.3 x 150 mm.A reversed-phase C18 HPLC column with fully porous ZORBAX particles.StableBond technology ensures stability at low pH.

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    150 mm

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5064-8264

ZORBAX 300SB-C18 5µm, 0.5 x 150 mm.A reversed-phase C18 HPLC column with fully porous ZORBAX particles.StableBond technology ensures stability at low pH.

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    150 mm

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5064-8266

ZORBAX 300SB-C18 5µm, 0.5 x 250 mm

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5064-8267

ZORBAX 300SB-C18 3.5µm, 0.3 x 150 mm.A reversed-phase C18 HPLC column with fully porous ZORBAX particles.StableBond technology ensures stability at low pH.

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    1 Each

  • Length:

    150 mm

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